Two-dimensional polyacrylamide gel electrophoresis (DE) and identification by TOF MS Proteins from Syno or Syno MEFs were separated by 2DE using non-linear pH 3-10 IPG strips for the first dimension and 4-20% gradient Tris-Glycine gels for the second dimension
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چکیده
Cruz Biotechnology), anti-FLAG (M2; Sigma Chemical Co., St. Louis, MO), anti-β-actin (AC-15; Sigma), and anti-α-tubulin (B-5-1-2; Sigma Chemical Co.) were used. Anti-Synoviolin monoclonal antibody (10Da) was reported previously (Amano et al., 2003). Cycloheximide (Sigma Chemical Co., 100μg/mL) was used for the chase experiment of p53. Camptothecine (Sigma, 0.5 mM), and actinomycin D (Sigma Chemical Co., 5 nM) were used 10
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